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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



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ID: 733157.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease
Authors:Lazzaretti, D.; Veith, K.; Kramer, K.; Basquin, C.; Urlaub, H.; Irion, U.; Bono, F.
Date of Publication (YYYY-MM-DD):2016-08
Title of Journal:Nat Struct Mol Biol
Volume:23
Issue / Number:8
Start Page:705
End Page:713
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. We present the crystal structure of Exu, which reveals a dimeric assembly with each monomer consisting of a 3'-5' EXO-like domain and a sterile alpha motif (SAM)-like domain. The catalytic site is degenerate and inactive. Instead, the EXO-like domain mediates dimerization and RNA binding. We show that Exu binds RNA directly in vitro, that the SAM-like domain is required for RNA binding activity and that Exu binds a structured element present in the bcd 3' untranslated region with high affinity. Through structure-guided mutagenesis, we show that Exu dimerization is essential for bcd localization. Our data demonstrate that Exu is a noncanonical RNA-binding protein with EXO-SAM-like domain architecture that interacts with its target RNA as a homodimer.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen/Arbeitsgruppe Bono
MPI für biophysikalische Chemie/AG Henning Urlaub
External Affiliations:Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany. Bioanalytics, Institute for Clinical Chemistry, University Medical Center Gottingen, Gottingen, Germany. Max Planck Institute of Biochemistry, Martinsried, Germany.
Identifiers:ISSN:1545-9985 (Electronic) 1545-9985 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27376588 [ID No:2]
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