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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents   history ID: 733157.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease Authors: Lazzaretti, D.; Veith, K.; Kramer, K.; Basquin, C.; Urlaub, H.; Irion, U.; Bono, F. Date of Publication (YYYY-MM-DD): 2016-08 Title of Journal: Nat Struct Mol Biol Volume: 23 Issue / Number: 8 Start Page: 705 End Page: 713 Review Status: Internal review Audience: Not Specified Abstract / Description: Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. We present the crystal structure of Exu, which reveals a dimeric assembly with each monomer consisting of a 3'-5' EXO-like domain and a sterile alpha motif (SAM)-like domain. The catalytic site is degenerate and inactive. Instead, the EXO-like domain mediates dimerization and RNA binding. We show that Exu binds RNA directly in vitro, that the SAM-like domain is required for RNA binding activity and that Exu binds a structured element present in the bcd 3' untranslated region with high affinity. Through structure-guided mutagenesis, we show that Exu dimerization is essential for bcd localization. Our data demonstrate that Exu is a noncanonical RNA-binding protein with EXO-SAM-like domain architecture that interacts with its target RNA as a homodimer. External Publication Status: published Document Type: Article Affiliations: MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen/Arbeitsgruppe Bono MPI für biophysikalische Chemie/AG Henning Urlaub External Affiliations: Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany. Bioanalytics, Institute for Clinical Chemistry, University Medical Center Gottingen, Gottingen, Germany. Max Planck Institute of Biochemistry, Martinsried, Germany. Identifiers: ISSN:1545-9985 (Electronic) 1545-9985 (Linking) %R 10.1... [ID No:1] URL:https://www.ncbi.nlm.nih.gov/pubmed/27376588 [ID No:2] The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.