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          Institute: MPI für Entwicklungsbiologie     Collection: Scientific Infrastructure Services     Display Documents



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ID: 733170.0, MPI für Entwicklungsbiologie / Scientific Infrastructure Services
Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains
Authors:Wild, R.; Gerasimaite, R.; Jung, J. Y.; Truffault, V.; Pavlovic, I.; Schmidt, A.; Saiardi, A.; Jessen, H. J.; Poirier, Y.; Hothorn, M.; Mayer, A.
Date of Publication (YYYY-MM-DD):2016-05-20
Title of Journal:Science
Volume:352
Issue / Number:6288
Start Page:986
End Page:990
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Phosphorus is a macronutrient taken up by cells as inorganic phosphate (P(i)). How cells sense cellular P(i) levels is poorly characterized. Here, we report that SPX domains--which are found in eukaryotic phosphate transporters, signaling proteins, and inorganic polyphosphate polymerases--provide a basic binding surface for inositol polyphosphate signaling molecules (InsPs), the concentrations of which change in response to P(i) availability. Substitutions of critical binding surface residues impair InsP binding in vitro, inorganic polyphosphate synthesis in yeast, and P(i) transport in Arabidopsis In plants, InsPs trigger the association of SPX proteins with transcription factors to regulate P(i) starvation responses. We propose that InsPs communicate cytosolic P(i) levels to SPX domains and enable them to interact with a multitude of proteins to regulate P(i) uptake, transport, and storage in fungi, plants, and animals.
Free Keywords:Arabidopsis/metabolism; Arabidopsis Proteins/chemistry/genetics; Crystallography, X-Ray; Cytosol/metabolism; *Homeostasis; Humans; Inositol/*metabolism; Phosphate Transport Proteins/*chemistry/genetics; Phosphorus/*metabolism; Polyphosphates/*metabolism; Protein Structure, Secondary/genetics; Protein Structure, Tertiary/genetics; Saccharomyces cerevisiae/metabolism; Transcription Factors/chemistry; Transferases (Other Substituted Phosphate Groups)/*chemistry/genetics
External Publication Status:published
Document Type:Article
Communicated by:Sigurdardottir
Affiliations:MPI für Entwicklungsbiologie
External Affiliations:Department of Biochemistry, University of Lausanne, Lausanne, Switzerland. Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland. Department of Biochemistry, Max Planck Institute for Developmental Biology, Tubingen, Germany. Department of Chemistry and Pharmacy, University of Zurich, Zurich, Switzerland. Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, UK. Department of Chemistry and Pharmacy, University of Zurich, Zurich, Switzerland. Institute of Organic Chemistry, Albert-Ludwigs-University Freiburg, Freiburg, Germany. Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland. yves.poirier@unil.ch michael.hothorn@unige.ch andreas.mayer@unil.ch. Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland. yves.poirier@unil.ch michael.hothorn@unige.ch andreas.mayer@unil.ch. Department of Biochemistry, University of Lausanne, Lausanne, Switzerland. yves.poirier@unil.ch michael.hothorn@unige.ch andreas.mayer@unil.ch.
Identifiers:ISSN:1095-9203 (Electronic) 0036-8075 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27080106 [ID No:2]
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