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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents

ID: 733216.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
LFQProfiler and RNP(xl): Open-Source Tools for Label-Free Quantification and Protein-RNA Cross-Linking Integrated into Proteome Discoverer
Authors:Veit, J.; Sachsenberg, T.; Chernev, A.; Aicheler, F.; Urlaub, H.; Kohlbacher, O.
Date of Publication (YYYY-MM-DD):2016-09-02
Title of Journal:J Proteome Res
Issue / Number:9
Start Page:3441
End Page:3448
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Modern mass spectrometry setups used in today's proteomics studies generate vast amounts of raw data, calling for highly efficient data processing and analysis tools. Software for analyzing these data is either monolithic (easy to use, but sometimes too rigid) or workflow-driven (easy to customize, but sometimes complex). Thermo Proteome Discoverer (PD) is a powerful software for workflow-driven data analysis in proteomics which, in our eyes, achieves a good trade-off between flexibility and usability. Here, we present two open-source plugins for PD providing additional functionality: LFQProfiler for label-free quantification of peptides and proteins, and RNP(xl) for UV-induced peptide-RNA cross-linking data analysis. LFQProfiler interacts with existing PD nodes for peptide identification and validation and takes care of the entire quantitative part of the workflow. We show that it performs at least on par with other state-of-the-art software solutions for label-free quantification in a recently published benchmark ( Ramus, C.; J. Proteomics 2016 , 132 , 51 - 62 ). The second workflow, RNP(xl), represents the first software solution to date for identification of peptide-RNA cross-links including automatic localization of the cross-links at amino acid resolution and localization scoring. It comes with a customized integrated cross-link fragment spectrum viewer for convenient manual inspection and validation of the results.
Free Keywords:analysis; computational; nodes; open-source software; workflow
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
MPI für biophysikalische Chemie
External Affiliations:Bioanalytics Group, University Medical Center Gottingen , 37077 Gottingen, Germany. Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry , 37077 Gottingen, Germany. Quantitative Biology Center, University of Tubingen , 72076 Tubingen, Germany. Biomolecular Interactions, Max Planck Institute for Developmental Biology , 72076 Tubingen, Germany.
Identifiers:ISSN:1535-3907 (Electronic) 1535-3893 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27476824 [ID No:2]
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