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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents

ID: 733230.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Learning from Heterogeneous Data Sources: An Application in Spatial Proteomics
Authors:Breckels, L. M.; Holden, S. B.; Wojnar, D.; Mulvey, C. M.; Christoforou, A.; Groen, A.; Trotter, M. W.; Kohlbacher, O.; Lilley, K. S.; Gatto, L.
Date of Publication (YYYY-MM-DD):2016-05
Title of Journal:PLoS Comput Biol
Issue / Number:5
Sequence Number of Article:e1004920
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Sub-cellular localisation of proteins is an essential post-translational regulatory mechanism that can be assayed using high-throughput mass spectrometry (MS). These MS-based spatial proteomics experiments enable us to pinpoint the sub-cellular distribution of thousands of proteins in a specific system under controlled conditions. Recent advances in high-throughput MS methods have yielded a plethora of experimental spatial proteomics data for the cell biology community. Yet, there are many third-party data sources, such as immunofluorescence microscopy or protein annotations and sequences, which represent a rich and vast source of complementary information. We present a unique transfer learning classification framework that utilises a nearest-neighbour or support vector machine system, to integrate heterogeneous data sources to considerably improve on the quantity and quality of sub-cellular protein assignment. We demonstrate the utility of our algorithms through evaluation of five experimental datasets, from four different species in conjunction with four different auxiliary data sources to classify proteins to tens of sub-cellular compartments with high generalisation accuracy. We further apply the method to an experiment on pluripotent mouse embryonic stem cells to classify a set of previously unknown proteins, and validate our findings against a recent high resolution map of the mouse stem cell proteome. The methodology is distributed as part of the open-source Bioconductor pRoloc suite for spatial proteomics data analysis.
Free Keywords:Algorithms; Animals; Arabidopsis; Computational Biology; Data Interpretation, Statistical; Drosophila; Embryonic Stem Cells/metabolism; Humans; Information Storage and Retrieval; Mass Spectrometry; Mice; Proteome/classification/*metabolism; Proteomics/*statistics & numerical data; Software; Subcellular Fractions/metabolism; Support Vector Machine
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom. Computer Laboratory, University of Cambridge, Cambridge, United Kingdom. Quantitative Biology Center, Universitat Tubingen, Tubingen, Germany. Celgene Institute for Translational Research Europe (CITRE), Sevilla, Spain. Center for Bioinformatics, Universitat Tubingen, Tubingen, Germany. Biomolecular Interactions, Max Planck Institute for Developmental Biology, Tubingen, Germany.
Identifiers:ISSN:1553-7358 (Electronic) 1553-734X (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27175778 [ID No:2]
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