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          Institute: MPI für bioanorganische Chemie     Collection: MPI für bioanorganische Chemie     Display Documents



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ID: 733844.0, MPI für bioanorganische Chemie / MPI für bioanorganische Chemie
Structural and functional characterization of the hydrogenase-maturation HydF protein
Authors:Caserta, Giorgio; Pecqueur, Ludovic; Adamska-Venkatesh, Agnieszka; Papini, Cecilia; Roy, Souvik; Artero, Vincent; Atta, Mohamed; Reijerse, Edward; Lubitz, Wolfgang; Fontecave, Marc
Language:English
Date of Publication (YYYY-MM-DD):2017
Title of Journal:Nature Chemical Biology
Journal Abbrev.:Nat.Chem.Biol.
Volume:13
Issue / Number:7
Start Page:779
End Page:784
Review Status:Internal review
Audience:Experts Only
Abstract / Description:[FeFe] hydrogenase (HydA) catalyzes interconversion between 2H(+) and H-2 at an active site composed of a [4Fe-4S] cluster linked to a 2Fe subcluster that harbors CO, CN- and azapropanedithiolate (adt(2-)) ligands. HydE, HydG and HydF are the maturases specifically involved in the biosynthesis of the 2Fe subcluster. Using ligands synthesized by HydE and HydG, HydF assembles a di-iron precursor of the 2Fe subcluster and transfers it to HydA for maturation. Here we report the first X-ray structure of HydF with its [4Fe-4S] cluster. The cluster is chelated by three cysteines and an exchangeable glutamate, which allows the binding of synthetic mimics of the 2Fe subcluster. [Fe-2(adt)(CO)(4)(CN)(2)](2-) is proposed to be the true di-iron precursor because, when bound to HydF, it matures HydA and displays features in Fourier transform infrared (FTIR) spectra that are similar to those of the native HydF active intermediate. A new route toward the generation of artificial hydrogenases, as combinations of HydF and such biomimetic complexes, is proposed on the basis of the observed hydrogenase activity of chemically modified HydF.
Comment of the Author/Creator:Date: 2017, JUL 2017
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:N. N.
Affiliations:MPI für bioanorganische Chemie
Identifiers:ISI:000403673700015 [ID No:1]
ISSN:1552-4450 [ID No:2]
DOI:10.1038/nchembio.2385 [ID No:3]
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