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          Institute: MPI für bioanorganische Chemie     Collection: MPI für bioanorganische Chemie     Display Documents



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ID: 733900.0, MPI für bioanorganische Chemie / MPI für bioanorganische Chemie
Novel mononuclear Cu (II) terpyridine complexes: Impact of fused ring thiophene and thiazole head groups towards DNA/BSA interaction, cleavage and antiproliferative activity on HepG2 and triple negative CAL-51 cell line
Authors:Manikandamathavan, V. M.; Thangaraj, M.; Weyhermüller, Thomas; Parameswari, R. P.; Punitha, V.; Murthy, N. N.; Nair, B. U.
Date of Publication (YYYY-MM-DD):2017
Title of Journal:European Journal of Medicinal Chemistry
Journal Abbrev.:Europ.J.Med.Chem.
Volume:135
Start Page:434
End Page:446
Review Status:Internal review
Audience:Experts Only
Abstract / Description:Two mononuclear copper (II) terpyridine complexes namely, [Cu(Btptpy) (ClO4)](ClO4) 1, and [Cu(Bttpy) (ClO4)](ClO4) 2, (Btptpy (L-1) = 4'-(Benzothiophene)-2,2':6',2"-terpyridine, Bttpy (L-2) = 4'-(Benzylthiazolyl)-2,2':6',2"-terpyridine) have been synthesized and characterized. Single crystal X-ray diffraction shows that, both ligands belong to monoclinic crystal system with space group P2(1)/c (L-1) and P2(1)/n (L-2). Absorption spectral titration, DNA melting study, circular dichroism and viscosity measurement reveal that, complex 1 and 2 bind with DNA through intercalation. In addition, interaction between the two copper (II) complexes and bovine serum albumin (BSA) has been studied by fluorescence titration, circular dichroism and their protease activity has been investigated using SDS-PAGE gel electrophoresis. Agarose (AGE) and SDS-PAGE gel electrophoresis reveals both complexes have good nucleolytic and proteolytic property in the presence of additive hydrogen peroxide. Both complexes shows remarkable cytotoxic property against triple negative CAL-51 human breast cancer cell line and hepatocellular carcinoma (HepG2) cancer cell lines and bears very less cytotoxicity towards liver normal cell line (Changs). DCF-DA and TBRAS assay also supported that complex 1 and 2 induces elevated level of reactive oxygen species (ROS) and oxidative stress in cancer cells than normal cell line. Furthermore, FACS analysis confirms complex 1 and 2 brings apoptosis by growth phase cell cycle arrest. (C) 2017 Elsevier-Masson SAS. All rights reserved.
Comment of the Author/Creator:Date: 2017, JUL 28 2017
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für bioanorganische Chemie
Identifiers:ISI:000403512100035 [ID No:1]
ISSN:0223-5234 [ID No:2]
DOI:10.1016/j.ejmech.2017.04.030 [ID No:3]
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