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          Institute: MPI für bioanorganische Chemie     Collection: MPI für bioanorganische Chemie     Display Documents



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ID: 734271.0, MPI für bioanorganische Chemie / MPI für bioanorganische Chemie
FRET in a Synthetic Flavin- and Bilin-binding Protein
Authors:Simon, Julian; Losi, Aba; Zhao, Kai-Hong; Gärtner, Wolfgang
Language:English
Date of Publication (YYYY-MM-DD):2017
Title of Journal:Photochemistry and Photobiology
Journal Abbrev.:Photochem.Photobiol.
Volume:93
Issue / Number:4
Start Page:1057
End Page:1062
Review Status:Internal review
Audience:Experts Only
Abstract / Description:The last decade has seen development and application of a large number of novel fluorescence-based techniques that have revolutionized fluorescence microscopy in life sciences. Preferred tags for such applications are genetically encoded fluorescent proteins (FP), mostly derivatives of the green fluorescent protein (GFP). Combinations of FPs with wavelength-separated absorption/fluorescence properties serve as excellent tools for molecular interaction studies, for example, protein-protein complexes or enzyme-substrate interactions, based on the FRET phenomenon (Forster resonance energy transfer). However, alternatives are requested for experimental conditions where FP proteins or FP couples are not or less efficiently applicable. We here report as a "proof of principle" a specially designed, non-naturally occurring protein (LG1) carrying a combination of a flavin-binding LOV- and a photochromic bilin-binding GAF domain and demonstrate a FRET process between both chromophores.
Comment of the Author/Creator:Date: 2017, JUL-AUG 2017
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:N. N.
Affiliations:MPI für bioanorganische Chemie
Identifiers:ISI:000404994000016 [ID No:1]
ISSN:0031-8655 [ID No:2]
DOI:10.1111/php.12707 [ID No:3]
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