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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents



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ID: 744094.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
The Endothelial Glycocalyx Controls Interactions of Quantum Dots with the Endothelium and Their Translocation across the Blood-Tissue Border
Authors:Uhl, B.; Hirn, S.; Immler, R.; Mildner, K.; Mockl, L.; Sperandio, M.; Brauchle, C.; Reichel, C. A.; Zeuschner, D.; Krombach, F.
Date of Publication (YYYY-MM-DD):2017-02-28
Title of Journal:ACS Nano
Volume:11
Issue / Number:2
Start Page:1498
End Page:1508
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Advances in the engineering of nanoparticles (NPs), which represent particles of less than 100 nm in one external dimension, led to an increasing utilization of nanomaterials for biomedical purposes. A prerequisite for their use in diagnostic and therapeutic applications, however, is the targeted delivery to the site of injury. Interactions between blood-borne NPs and the vascular endothelium represent a critical step for nanoparticle delivery into diseased tissue. Here, we show that the endothelial glycocalyx, which constitutes a glycoprotein-polysaccharide meshwork coating the luminal surface of vessels, effectively controls interactions of carboxyl-functionalized quantum dots with the microvascular endothelium. Glycosaminoglycans of the endothelial glycocalyx were found to physically cover endothelial adhesion and signaling molecules, thereby preventing endothelial attachment, uptake, and translocation of these nanoparticles through different layers of the vessel wall. Conversely, degradation of the endothelial glycocalyx promoted interactions of these nanoparticles with microvascular endothelial cells under the pathologic condition of ischemia-reperfusion, thus identifying the injured endothelial glycocalyx as an essential element of the blood-tissue border facilitating the targeted delivery of nanomaterials to diseased tissue.
Free Keywords:*blood-tissue border; *endothelial glycocalyx; *multiphoton in vivo microscopy; *nanoparticles; *nanoparticle-endothelium interactions; *quantum dots; *translocation
External Publication Status:published
Document Type:Article
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Electron Microscopy Unit, Max Planck Institute for Molecular Biomedicine , 48149 Munster, Germany. Department of Chemistry and Center for NanoScience (CeNS), Ludwig-Maximilians-Universitat Munchen , 81377 Munich, Germany. Department of Otorhinolaryngology, Head and Neck Surgery, Klinikum der Universitat Munchen, Ludwig-Maximilians-Universitat Munchen , 81377 Munich, Germany.
Identifiers:ISSN:1936-086X (Electronic) 1936-0851 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/28135073 [ID No:2]
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