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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents



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ID: 744095.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
Blockage of the Epithelial-to-Mesenchymal Transition Is Required for Embryonic Stem Cell Derivation
Authors:Totonchi, M.; Hassani, S. N.; Sharifi-Zarchi, A.; Tapia, N.; Adachi, K.; Arand, J.; Greber, B.; Sabour, D.; Arauzo-Bravo, M. J.; Walter, J.; Pakzad, M.; Gourabi, H.; Scholer, H. R.; Baharvand, H.
Date of Publication (YYYY-MM-DD):2017-10-10
Title of Journal:Stem Cell Reports
Volume:9
Issue / Number:4
Start Page:1275
End Page:1290
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Pluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ESCs, through inhibition of the MEK and TGF-beta pathways. This regimen provides a platform for dissecting the molecular mechanism of ESC derivation. Via temporal gene expression analysis, we reveal key genes involved in the ICM to ESC transition. We found that DNA methyltransferases play a pivotal role in efficient ESC generation. We further observed a tight correlation between ESCs and preimplantation epiblast cell-related genes and noticed that fundamental events such as epithelial-to-mesenchymal transition blockage play a key role in launching the ESC self-renewal program. Our study provides a time course transcriptional resource highlighting the dynamics of the gene regulatory network during the ICM to ESC transition.
Free Keywords:Animals; Biomarkers; Blastocyst Inner Cell Mass/cytology; *Cell Differentiation/drug effects/genetics; DNA Methylation; Embryonic Stem Cells/*cytology/*metabolism; *Epithelial-Mesenchymal Transition/drug effects/genetics; Gene Expression Profiling; Gene Expression Regulation, Developmental; Mice; RNA Interference; Transcriptome; *emt; *ESC derivation; *mouse
External Publication Status:published
Document Type:Article
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran; Department of Developmental Biology, University of Science and Culture, Tehran, Iran. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran; Chitsaz Lab, Department of Computer Science, Colorado State University, Fort Collins 80523, CO, USA. Institute of Biomedicine of Valencia, Spanish National Research Council, Jaime Roig 11, 46010 Valencia, Spain. Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany. University of Saarland, FR 8.3, Biological Sciences, Genetics/Epigenetics, Campus A2.4, 66123 Saarbrucken, Germany. Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany; Chemical Genomics Centre of the Max Planck Society, Dortmung, Germany. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran; Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany. Group of Computational Biology and Systems Biomedicine, Biodonostia Health Research Institute, 20014 San Sebastian, Spain. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran; Department of Developmental Biology, University of Science and Culture, Tehran, Iran. Electronic address: baharvand@royaninstitute.org.
Identifiers:ISSN:2213-6711 (Electronic) 2213-6711 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/28919260 [ID No:2]
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