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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents

ID: 744096.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
Expression of receptor-type protein tyrosine phosphatase in developing and adult renal vasculature
Authors:Takahashi, K.; Kim, R.; Lauhan, C.; Park, Y.; Nguyen, N. G.; Vestweber, D.; Dominguez, M. G.; Valenzuela, D. M.; Murphy, A. J.; Yancopoulos, G. D.; Gale, N. W.; Takahashi, T.
Date of Publication (YYYY-MM-DD):2017
Title of Journal:PLoS ONE
Issue / Number:5
Start Page:e0177192
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Renal vascular development is a coordinated process that requires ordered endothelial cell proliferation, migration, intercellular adhesion, and morphogenesis. In recent decades, studies have defined the pivotal role of endothelial receptor tyrosine kinases (RPTKs) in the development and maintenance of renal vasculature. However, the expression and the role of receptor tyrosine phosphatases (RPTPs) in renal endothelium are poorly understood, though coupled and counterbalancing roles of RPTKs and RPTPs are well defined in other systems. In this study, we evaluated the promoter activity and immunolocalization of two endothelial RPTPs, VE-PTP and PTPmu, in developing and adult renal vasculature using the heterozygous LacZ knock-in mice and specific antibodies. In adult kidneys, both VE-PTP and PTPmu were expressed in the endothelium of arterial, glomerular, and medullary vessels, while their expression was highly limited in peritubular capillaries and venous endothelium. VE-PTP and PTPmu promoter activity was also observed in medullary tubular segments in adult kidneys. In embryonic (E12.5, E13.5, E15.5, E17.5) and postnatal (P0, P3, P7) kidneys, these RPTPs were expressed in ingrowing renal arteries, developing glomerular microvasculature (as early as the S-shaped stage), and medullary vessels. Their expression became more evident as the vasculatures matured. Peritubular capillary expression of VE-PTP was also noted in embryonic and postnatal kidneys. Compared to VE-PTP, PTPmu immunoreactivity was relatively limited in embryonic and neonatal renal vasculature and evident immunoreactivity was observed from the P3 stage. These findings indicate 1) VE-PTP and PTPmu are expressed in endothelium of arterial, glomerular, and medullary renal vasculature, 2) their expression increases as renal vascular development proceeds, suggesting that these RPTPs play a role in maturation and maintenance of these vasculatures, and 3) peritubular capillary VE-PTP expression is down-regulated in adult kidneys, suggesting a role of VE-PTP in the development of peritubular capillaries.
Free Keywords:Animals; Endothelium, Vascular/*metabolism; Kidney/*metabolism; Mice; Phosphorylation/genetics; Promoter Regions, Genetic/genetics; Protein Tyrosine Phosphatases/genetics/metabolism; Receptor Protein-Tyrosine Kinases/*genetics/*metabolism
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:University of Oklahoma College of Medicine, Oklahoma City, Oklahoma, United States of America. Max-Planck-Institute of Molecular Biomedicine, Munster, Germany. Regeneron Pharmaceuticals, Inc., Tarrytown, New York, United States of America. Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America.
Identifiers:ISSN:1932-6203 (Electronic) 1932-6203 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/28542220 [ID No:2]
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