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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents



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ID: 744099.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
Genetic dissection of endothelial transcriptional activity of zebrafish aryl hydrocarbon receptors (AHRs)
Authors:Sugden, W. W.; Leonardo-Mendonca, R. C.; Acuna-Castroviejo, D.; Siekmann, A. F.
Date of Publication (YYYY-MM-DD):2017
Title of Journal:PLoS ONE
Volume:12
Issue / Number:8
Start Page:e0183433
Review Status:Internal review
Audience:Not Specified
Abstract / Description:The aryl hydrocarbon receptor (AHR) is a basic helix-loop-helix transcription factor conserved across phyla from flies to humans. Activated by a number of endogenous ligands and environmental toxins, studies on AHR function and gene regulation have largely focused on a toxicological perspective relating to aromatic hydrocarbons generated by human activities and the often-deleterious effects of exposure on vertebrates mediated by AHR activation. A growing body of work has highlighted the importance of AHR in physiologic processes, including immune cell differentiation and vascular patterning. Here we dissect the contribution of the 3 zebrafish AHRs, ahr1a, ahr1b and ahr2, to endothelial cyp1a1/b1 gene regulation under physiologic conditions and upon exposure to the AHR ligand Beta-naphthoflavone. We show that in fish multiple AHRs are functional in the vasculature, with vessel-specific differences in the ability of ahr1b to compensate for the loss of ahr2 to maintain AHR signaling. We further provide evidence that AHR can regulate the expression of the chemokine receptor cxcr4a in endothelial cells, a regulatory mechanism that may provide insight into AHR function in the endothelium.
Free Keywords:Animals; Cytochrome P-450 Enzyme System/genetics; Endothelium, Vascular/*metabolism; Gene Expression Regulation, Developmental; In Situ Hybridization; Mutagenesis; Receptors, Aryl Hydrocarbon/*genetics; *Transcription, Genetic; Zebrafish/embryology/*genetics
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Cells-in-Motion Cluster of Excellence (EXC 1003 -CiM), University of Muenster, Muenster, Germany. Universidade Atlantica, Fabrica da Polvora de Barcarena, Barcarena, Portugal. Instituto de Biotecnologia, Centro de Investigacion Biomedica, Parque Tecnologico de Ciencias de la Salud, Universidad de Granada, Granada, Spain. Unidad de Gestion Clinica de Laboratorios Clinicos, CIBER de Fragilidad y Envejecimiento, Ibs.Granada, Complejo Hospitalario de Granada, Granada, Spain.
Identifiers:ISSN:1932-6203 (Electronic) 1932-6203 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/28817646 [ID No:2]
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