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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents



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ID: 744135.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
Emergence of CD43-Expressing Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells
Authors:Kessel, K. U.; Bluemke, A.; Scholer, H. R.; Zaehres, H.; Schlenke, P.; Dorn, I.
Date of Publication (YYYY-MM-DD):2017-06
Title of Journal:Transfusion Medicine and Hemotherapy : Offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie
Volume:44
Issue / Number:3
Start Page:143
End Page:150
Review Status:Internal review
Audience:Not Specified
Abstract / Description:BACKGROUND: The ex vivo generation of human hematopoietic stem cells (HSCs) with long-term repopulating capacity and multi-lineage differentiation potential represents the holy grail of hematopoiesis research. In principle, human induced pluripotent stem cells (hiPSCs) provide the tool for both studying molecular mechanisms of hematopoietic development and the ex vivo production of 'true' HSCs for transplantation purposes and lineage-specific cells, e.g. red blood cells, for transfusion purposes. CD43-expressing cells have been reported as the first hematopoietic cells during development, but whether or not these possess multilineage differentiation and long-term engraftment potential is incompletely understood. METHODS: We performed ex vivo generation of hematopoietic cells from hiPSCs using an embryoid body(EB)-based, xeno-product-free differentiation protocol. We investigated the multilineage differentiation potential of different FACS-sorted CD43-expressing cell subsets by colony-forming assays in semisolid media. Further, erythroid differentiation was investigated in more detail using established protocols. RESULTS: By using CD43, we were able to measure hematopoietic induction efficiency during hiPSC-derived EB differentiation. Further, we determined CD43+ cells as the cell population of origin for in vitro erythropoiesis. Furthermore, colony formation demonstrates that the multipotent hematopoietic stem and progenitor cell fraction is particulary enriched in the CD43(hi) CD45+ population.
Free Keywords:Embryoid body; Hematopoiesis; Hematopoietic stem cells; Induced pluripotent stem cells
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Medical Faculty, Westphalian Wilhelms University Munster, Munster, Germany. Department of Anatomy and Molecular Embryology, Medical Faculty, Ruhr-University Bochum, Bochum, Germany. Medical University Graz; Department for Blood Group Serology and Transfusion Medicine, Graz, Austria.
Identifiers:ISSN:1660-3796 (Print) 1660-3796 (Linking) %R 10.1159/0... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/28626365 [ID No:2]
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