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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents

ID: 744143.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
Wnt signaling positively regulates endothelial cell fate specification in the Fli1a-positive progenitor population via Lef1
Authors:Hübner, K.; Grassme, K. S.; Rao, J.; Wenke, N. K.; Zimmer, C. L.; Korte, L.; Müller, K.; Sumanas, S.; Greber, B.; Herzog, W.
Date of Publication (YYYY-MM-DD):2017-08-12
Title of Journal:Dev Biol
Start Page:142
End Page:155
Review Status:Internal review
Audience:Not Specified
Abstract / Description:During vertebrate embryogenesis, vascular endothelial cells (ECs) and primitive erythrocytes become specified within close proximity in the posterior lateral plate mesoderm (LPM) from a common progenitor. However, the signaling cascades regulating the specification into either lineage remain largely elusive. Here, we analyze the contribution of beta-catenin dependent Wnt signaling to EC and erythrocyte specification during zebrafish embryogenesis. We generated novel beta-catenin dependent Wnt signaling reporters which, by using destabilized fluorophores (Venus-Pest, dGFP), specifically allow us to detect Wnt signaling responses in narrow time windows as well as in spatially restricted domains, defined by Cre recombinase expression (Tg(axin2BAC:Venus-Pest)mu288; Tg(14TCF:loxP-STOP-loxP-dGFP)mu202). We therefore can detect beta-catenin dependent Wnt signaling activity in a subset of the Fli1a-positive progenitor population. Additionally, we show that mesodermal Wnt3a-mediated signaling via the transcription factor Lef1 positively regulates EC specification (defined by kdrl expression) at the expense of primitive erythrocyte specification (defined by gata1 expression) in zebrafish embryos. Using mesoderm derived from human embryonic stem cells, we identified the same principle of Wnt signaling dependent EC specification in conjunction with auto-upregulation of LEF1. Our data indicate a novel role of beta-catenin dependent Wnt signaling in regulating EC specification during vasculogenesis.
Free Keywords:Mesoderm; Primitive hematopoiesis; Vasculogenesis; Wnt reporter; Zebrafish
External Publication Status:published
Document Type:Article
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:University of Muenster, Muenster, Germany. Max Planck Institute for Molecular Biomedicine, Muenster, Germany; Chemical Genomics Centre of the Max Planck Society, Dortmund, Germany. Ulm University, Ulm, Germany. Max Planck Institute for Molecular Biomedicine, Muenster, Germany. Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. University of Muenster, Muenster, Germany; Cells-in-Motion Cluster of Excellence (EXC 1003 - CiM), University of Muenster, Germany; Max Planck Institute for Molecular Biomedicine, Muenster, Germany. Electronic address: wiebke.herzog@uni-muenster.de.
Identifiers:ISSN:1095-564X (Electronic) 0012-1606 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/28811218 [ID No:2]
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