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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents

ID: 744151.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
A Novel Cervical Spinal Cord Window Preparation Allows for Two-Photon Imaging of T-Cell Interactions with the Cervical Spinal Cord Microvasculature during Experimental Autoimmune Encephalomyelitis
Authors:Haghayegh Jahromi, N.; Tardent, H.; Enzmann, G.; Deutsch, U.; Kawakami, N.; Bittner, S.; Vestweber, D.; Zipp, F.; Stein, J. V.; Engelhardt, B.
Date of Publication (YYYY-MM-DD):2017
Title of Journal:Front Immunol
Start Page:406
Review Status:Internal review
Audience:Not Specified
Abstract / Description:T-cell migration across the blood-brain barrier (BBB) is a crucial step in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). Two-photon intravital microscopy (2P-IVM) has been established as a powerful tool to study cell-cell interactions in inflammatory EAE lesions in living animals. In EAE, central nervous system inflammation is strongly pronounced in the spinal cord, an organ in which 2P-IVM imaging is technically very challenging and has been limited to the lumbar spinal cord. Here, we describe a novel spinal cord window preparation allowing to use 2P-IVM to image immune cell interactions with the cervical spinal cord microvascular endothelium during EAE. We describe differences in the angioarchitecture of the cervical spinal cord versus the lumbar spinal cord, which will entail different hemodynamic parameters in these different vascular beds. Using T cells as an example, we demonstrate the suitability of this novel methodology in imaging the post-arrest multistep T-cell extravasation across the cervical spinal cord microvessels. The novel methodology includes an outlook to the analysis of the cellular pathway of T-cell diapedesis across the BBB by establishing visualization of endothelial junctions in this vascular bed.
Free Keywords:T-cell migration; blood-brain barrier; cervical spinal cord window; experimental autoimmune encephalomyelitis; two-photon intravital microscopy
External Publication Status:published
Document Type:Article
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Max Planck Institute of Neurobiology, Martinsried, Germany. Institute of Clinical Neuroimmunology, Biomedical Center and University Hospital, Ludwig-Maximilians University of Munich, Martinsried, Germany. Focus Program Translational Neuroscience (FTN) and Immunotherapy (FZI), Rhine Main Neuroscience Network (rmn2), University Medical Center of the Johannes Gutenberg University, Mainz, Germany. Max-Planck-Institute of Molecular Biomedicine, Munster, Germany.
Identifiers:ISSN:1664-3224 (Print) 1664-3224 (Linking) %R 10.3389/f... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/28443093 [ID No:2]
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