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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2018 (publ. 2017, arch)     Display Documents



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ID: 744153.0, MPI für molekulare Biomedizin / Jahrbuch 2018 (publ. 2017, arch)
Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations
Authors:Goudarzi, M.; Tarbashevich, K.; Mildner, K.; Begemann, I.; Garcia, J.; Paksa, A.; Reichman-Fried, M.; Mahabaleshwar, H.; Blaser, H.; Hartwig, J.; Zeuschner, D.; Galic, M.; Bagnat, M.; Betz, T.; Raz, E.
Date of Publication (YYYY-MM-DD):2017-12-04
Title of Journal:Dev Cell
Volume:43
Issue / Number:5
Start Page:577
End Page:587 e575
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Cell migration is essential for morphogenesis, organ formation, and homeostasis, with relevance for clinical conditions. The migration of primordial germ cells (PGCs) is a useful model for studying this process in the context of the developing embryo. Zebrafish PGC migration depends on the formation of cellular protrusions in form of blebs, a type of protrusion found in various cell types. Here we report on the mechanisms allowing the inflation of the membrane during bleb formation. We show that the rapid expansion of the protrusion depends on membrane invaginations that are localized preferentially at the cell front. The formation of these invaginations requires the function of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape changes performed by migrating cells. Inhibiting the formation and release of the invaginations strongly interfered with bleb formation, cell motility, and the ability of the cells to reach their target.
Free Keywords:Actins/metabolism; Animals; Cell Membrane/*metabolism; Cell Membrane Structures/metabolism; Cell Movement/*physiology; Cell Shape/*physiology; Cell Surface Extensions/metabolism; Germ Cells/*cytology/metabolism; *Zebrafish/metabolism; *N-bar; *Pgc; *bleb; *cell migration; *membrane; *primordial germ cells; *zebrafish
External Publication Status:published
Document Type:Article
Communicated by:MPI für molekulare Biomedizin
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Electron Microscopy Unit, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany. Workgroup Nanoforces in Cells, Institute of Medical Physics und Biophysics, DFG Cluster of Excellence 'Cells in Motion' (EXC 1003), Robert-Koch-Strasse 31, 48149 Munster, Germany. Department of Cell Biology, Duke University, 333B Nanaline Duke Building, Box 3709, Durham, NC 27710, USA. Germ Cell Development, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37070 Gottingen, Germany. Institute for Cell Biology, ZMBE, Von-Esmarch-Strasse 56, 48149 Munster, Germany. Electronic address: erez.raz@uni-muenster.de.
Identifiers:ISSN:1878-1551 (Electronic) 1534-5807 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/29173819 [ID No:2]
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