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          Document History for Document ID 17083

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Document Version Version Comment Date Status
17083.0 [No comment] 26.10.2010 13:59 Released

ID: 17083.0, MPI für biophysikalische Chemie / NanoBiophotonik (Dr. Stefan Hell)
Phase determination in interference-based superresolving microscopes through critical frequency analysis
Authors:Hell, S. W.; Blanca, C. M.; Bewersdorf, J.
Language:English
Date of Publication (YYYY-MM-DD):2002-06-02
Title of Journal:Optics Letters
Volume:27
Issue / Number:11
Start Page:888
End Page:890
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Utilizing the interference of wave fronts of two opposing lenses, 4Pi-confocal and (IM)-M-5 microscopy improve the axial resolution of far-field fluorescence microscopy as much as threefold to sevenfold. However, establishing the phase difference of the wave fronts in the sample is a problem yet to be solved. Here we show that the phase difference is encoded in the microscope's transfer of the spatial frequencies that match the distance of the interference peaks. As a result the phase difference is readily extracted through a Fourier transform of the image. Our method is relevant to all microscopes that exploit the interference of counterpropagating waves to improve the axial and the lateral resolution. (C) 2002 Optical Society of America
Comment of the Author/Creator:Date: 2002, JUN 1
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Stefan Hell / 200
Identifiers:URL:http://www.opticsinfobase.org/viewmedia.cfm?uri=ol...