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Document Version Version Comment Date Status
206709.0 [No comment] 14.05.2009 11:15 Released

ID: 206709.0, MPI für biophysikalische Chemie / Molekulare Biologie (Dr. Thomas M. Jovin)
Diffusion of microspheres in sealed and open microarrays
Authors:Rieger, B.; Dietrich, H. R. C.; van den Doel, R. L.; van Vliet, L. J.
Date of Publication (YYYY-MM-DD):2004-12
Title of Journal:Microscopy Research and Technique
Start Page:218
End Page:225
Sequence Number of Article:DOI 10.1002/jemt.20128
Copyright:© 2005 WILEY-LISS, INC.
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In several experiments, we study the diffusion of microspheres with different radii in microarrays filled with a variety of aqueous solutions of ethylene glycol. We study diffusion in open and closed (sealed) microarrays. In sealed nanoliter wells, the tracers show pure diffusion, whereas in open reactors, a radial outward-directed evaporation-induced liquid flow is superimposed onto the diffusion. In general, one of the following quantities can be calculated if the others are known: the temperature, the viscosity of the medium, the radius of the microbeads, or the diffusion constant. The estimated diffusion constants in closed microarrays are in good agreement with theoretical predictions based on the Brownian motion. We monitor the motion of the microbeads under a microscope and extract their paths in time from the digital recordings. Ambiguous paths due to the crossing of two trajectories can be detected. We show that low microsphere concentrations or high viscosities do not hamper a robust estimation of the diffusion parameters.
Free Keywords:Brownian motion; diffusion; mean square displacement; micro arrays; microarrays; micro beads
Last Change of the Resource (YYYY-MM-DD):2005-01-10
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Thomas Jovin / 060
External Affiliations:Quantitative Imaging Group, Faculty of Applied Sciences, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands; Roosevelt Academy, Lange Noordstraat 1, 4330 AB Middelburg, The Netherlands