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271548.0 [No comment] 30.05.2006 13:39 Released

ID: 271548.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling
Authors:Gutjahr, Claudia; Murphy, Derek; Lueking, Angelika; Koenig, Andrea; Janitz, Michal; O'Brien, John; Korn, Bernhard; Horn, Sabine; Lehrach, Hans; Cahill, Dolores J.
Date of Publication (YYYY-MM-DD):2005-01-07
Title of Journal:Genomics
Issue / Number:3
Start Page:285
End Page:296
Copyright:© 2004 Elsevier Inc.
Review Status:not specified
Audience:Experts Only
Abstract / Description:The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (TH1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse TH1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.
Free Keywords:TH1; cDNA expression library; Automation; Mouse; High-density protein arrays; Microarrays; Protein chips; Serum profiling; SLE; Autoantibody
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Centre for Human Proteomics, Royal College of Surgeons in Ireland, 123, St. Stephen's Green, Dublin 2, Ireland;
Ruhr-University Bochum, Medical Proteome Center, Universitätsstrasse 150, D-44780 Bochum, Germany;
Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, 123, St. Stephen's Green, Dublin 2, Ireland;
Resource Center for Genome Research, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany;
PROTAGEN AG, Emil-Figge-Strasse 76a, 44227 Dortmund, Germany
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