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Document Version Version Comment Date Status
35702.0 [No comment] 15.12.2010 11:56 Released

ID: 35702.0, MPI für Evolutionsbiologie / Ecophysiology
A reduced model of the fluorescence from the cyanobacterial photosynthetic apparatus designed for the in situ detection of cyanobacteria
Authors:Beutler, M.; Wiltshire, K. H.; Arp, M.; Kruse, J.; Reineke, C.; Moldaenke, C.; Hansen, U. P.
Date of Publication (YYYY-MM-DD):2003-04-18
Title of Journal:Biochimica et Biophysica Acta-Bioenergetics
Journal Abbrev.:Biochim. Biophys. Acta-Bioenerg.
Issue / Number:1
Start Page:33
End Page:46
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Fluorometric determination of the chlorophyll (Chl) content of cyanobacteria is impeded by the unique structure of their photosynthetic apparatus, i.e., the phycobilisomes (PBSs) in the light-harvesting antennae. The problems are caused by the variations in the ratio of the pigment PC to Chl a resulting from adaptation to varying environmental conditions. In order to include cyanobacteria in fluorometric analysis of algae, a simplified energy distribution model describing energy pathways in the cyanobacterial photosynthetic apparatus was conceptualized. Two sets of mathematical equations were derived from this model and tested. Fluorescence of cyanobacteria was measured with a new fluorometer at seven excitation wavelength ranges and at three detection channels (650, 685 and 720 nm) in vivo. By employing a new fit procedure, we were able to correct for variations in the cyanobacterial fluorescence excitation spectra and to account for other phytoplankton signals. The effect of energy-state transitions on the PC fluorescence emission of PBSs was documented. The additional use of the PC fluorescence signal in combination with our recently developed mathematical approach for phytoplankton analysis based on Chl fluorescence spectroscopy allows a more detailed study of cyanobacteria and other phytoplankton in vivo and in situ. (C) 2003 Elsevier Science B.V. All rights reserved.
Free Keywords:phycobilisome; energy distribution model; phycocyanin; cyanobacteria; fluorescence; phytoplankton
Comment of the Author/Creator:Date: 2003, APR 18
External Publication Status:published
Document Type:Article
Affiliations:MPI für Evolutionsbiologie/Abt. Ökophysiologie
External Affiliations:Univ Kiel, ZBM, D-24098 Kiel, Germany; Stiftung Alfred Wegner Inst, Biol Anstalt Helgoland, D-27483 Helgoland, Germany; Bbe Moldaenke, D-24119 Kiel, Germany
Relations:Is Version Of-EDOCID:111901
Identifiers:ISI:000182280600005 [ID No:1]
ISSN:0005-2728 [ID No:2]
DOI:10.1016/S0005-2728(03)00022-7 [ID No:3]
LOCALID:2211/S 37945 [Listen-Nummer/S-Nummer]
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