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Document Version Version Comment Date Status
400792.0 [No comment] 31.05.2011 15:43 Released

ID: 400792.0, MPI für Neurobiologie / Systems and Computational Neurobiology
A genetically encoded calcium indicator for chronic in vivo two-photon imaging
Authors:Mank, M.; Santos, A. F.; Direnberger, S.; Mrsic-Flogel, T. D.; Hofer, S. B.; Stein, V.; Hendel, T.; Reiff, D. F.; Levelt, C.; Borst, A.; Bonhoeffer, T.; Hübener, M.; Griesbeck, O.
Date of Publication (YYYY-MM-DD):2008-09
Title of Journal:Nature Methods
Journal Abbrev.:Nat. Methods
Issue / Number:9
Start Page:805
End Page:811
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Neurons in the nervous system can change their functional properties over time. At present, there are no techniques that allow reliable monitoring of changes within identified neurons over repeated experimental sessions. We increased the signal strength of troponin C-based calcium biosensors in the low-calcium regime by mutagenesis and domain rearrangement within the troponin C calcium binding moiety to generate the indicator TN-XXL. Using in vivo two-photon ratiometric imaging, we show that TN-XXL exhibits enhanced fluorescence changes in neurons of flies and mice. TN-XXL could be used to obtain tuning curves of orientation-selective neurons in mouse visual cortex measured repeatedly over days and weeks. Thus, the genetically encoded calcium indicator TN-XXL allows repeated imaging of response properties from individual, identified neurons in vivo, which will be crucial for gaining new insights into cellular mechanisms of plasticity, regeneration and disease.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Neurobiologie/Systems and Computational Neurobiology (Borst)/Cellular Dynamics (Griesbeck)
MPI für Neurobiologie/Cellular and Systems Neurobiology (Bonhoeffer)
MPI für Neurobiologie/Cellular and Systems Neurobiology (Bonhoeffer)/Synaptic Receptor Trafficking (Stein)
External Affiliations:Royal Netherlands Acad Arts & Sci, Netherlands Inst Neurosci, NL-1105 BA Amsterdam, Netherlands.
Identifiers:ISI:000258912700014 [ID No:1]
ISSN:1548-7091 [ID No:2]