Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Document History for Document ID 460660

Back to latest document version
Document Version Version Comment Date Status
460660.0 [No comment] 20.03.2013 12:06 Released

ID: 460660.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Cis-regulatory changes at FLOWERING LOCUS T mediate natural variation in flowering responses of Arabidopsis thaliana
Authors:Schwartz, C.; Balasubramanian, S.; Warthmann, N.; Michael, T. P.; Lempe, J.; Sureshkumar, S.; Kobayashi, Y.; Maloof, J. N.; Borevitz, J. O.; Chory, J.; Weigel, D.
Date of Publication (YYYY-MM-DD):2009-08-03
Title of Journal:Genetics
Volume:183
Issue / Number:2
Start Page:723
End Page:732
Review Status:not specified
Audience:Not Specified
Abstract / Description:Flowering time, a critical adaptive trait, is modulated by several environmental cues. These external signals converge on a small set of genes that in turn mediate the flowering response. Mutant analysis and subsequent molecular studies have revealed that one of these integrator genes, FLOWERING LOCUS T (FT), responds to photoperiod and temperature cues, two environmental parameters that greatly influence flowering time. As the central player in the transition to flowering, the protein coding sequence of FT and its function are highly conserved across species. Using QTL mapping with a new advanced intercross-recombinant inbred line (AI-RIL) population, we show that a QTL tightly linked to FT contributes to natural variation in the flowering response to the combined effects of photoperiod and ambient temperature. Using Heterogeneous Inbred Families (HIF) and introgression lines, we fine map the QTL to a 6.7 kb fragment in the FT promoter. We confirm by quantitative complementation that FT has differential activity in the two parental strains. Further support for FT underlying the QTL comes from a new approach, quantitative knockdown with artificial microRNAs (amiRNAs). Consistent with the causal sequence polymorphism being in the promoter, we find that the QTL affects FT expression. Taken together, these results indicate that allelic variation at pathway integrator genes such as FT can underlie phenotypic variability and that this may be achieved through cis-regulatory changes.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:%G Eng
Identifiers:ISSN:0016-6731 (Print) %R genetics.109.104984 [pii] 10.... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]