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          Document History for Document ID 546715

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Document Version Version Comment Date Status
546715.0 Automatic journal name synchronization 19.03.2011 20:15 Released

ID: 546715.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2010-arch
Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis.
Authors:Katis, Vittorio L; Lipp, Jesse J.; Imre, Richard; Bogdanova, Aliona; Okaz, Elwy; Habermann, Bianca; Mechtler, Karl; Nasmyth, Kim; Zachariae, Wolfgang
Date of Publication (YYYY-MM-DD):2010
Title of Journal:Developmental Cell
Volume:18
Issue / Number:3
Start Page:397
End Page:409
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:During meiosis, two rounds of chromosome segregation after a single round of DNA replication produce haploid gametes from diploid precursors. At meiosis I, maternal and paternal kinetochores are pulled toward opposite poles, and chiasmata holding bivalent chromosomes together are resolved by cleavage of cohesin's alpha-kleisin subunit (Rec8) along chromosome arms. This creates dyad chromosomes containing a pair of chromatids joined solely by cohesin at centromeres that had resisted cleavage. The discovery that centromeric Rec8 is protected from separase during meiosis I by shugoshin/MEI-S332 proteins that bind PP2A phosphatase suggests that phosphorylation either of separase or cohesin may be necessary for Rec8 cleavage. We show here that multiple phosphorylation sites within Rec8 as well as two different kinases, casein kinase 1delta/epsilon (CK1delta/epsilon) and Dbf4-dependent Cdc7 kinase (DDK), are required for Rec8 cleavage and meiosis I nuclear division. Rec8 with phosphomimetic mutations is no longer protected from separase at centromeres and is cleaved even when the two kinases are inhibited. Our data suggest that PP2A protects centromeric cohesion by opposing CK1delta/epsilon- and DDK-dependent phosphorylation of Rec8.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4339