Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Quick Search
My eDoc
Support Wiki
Direct access to
document ID:

          Document History for Document ID 561536

Back to latest document version
Document Version Version Comment Date Status
561536.0 [No comment] 20.03.2013 11:45 Released

ID: 561536.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
The bacterial N-end rule pathway: expect the unexpected
Authors:Dougan, D. A.; Truscott, K. N.; Zeth, K.
Date of Publication (YYYY-MM-DD):2010-05
Title of Journal:Molecular Microbiology
Issue / Number:3
Start Page:545
End Page:558
Review Status:not specified
Audience:Not Specified
Abstract / Description:The N-end rule pathway is a highly conserved process that operates in many different organisms. It relates the metabolic stability of a protein to its N-terminal amino acid. Consequently, amino acids are described as either 'stabilizing' or 'destabilizing'. Destabilizing residues are organized into three hierarchical levels: primary, secondary, and in eukaryotes - tertiary. Secondary and tertiary destabilizing residues act as signals for the post-translational modification of the target protein, ultimately resulting in the attachment of a primary destabilizing residue to the N-terminus of the protein. Regardless of their origin, proteins containing N-terminal primary destabilizing residues are recognized by a key component of the pathway. In prokaryotes, the recognition component is a specialized adaptor protein, known as ClpS, which delivers target proteins directly to the ClpAP protease for degradation. In contrast, eukaryotes use a family of E3 ligases, known as UBRs, to recognize and ubiquitylate their substrates resulting in their turnover by the 26S proteasome. While the physiological role of the N-end rule pathway is largely understood in eukaryotes, progress on the bacterial pathway has been slow. However, new interest in this area of research has invigorated several recent advances, unlocking some of the secrets of this unique proteolytic pathway in prokaryotes.
Free Keywords:Amino Acid Motifs; Bacteria/chemistry/genetics/*metabolism; Bacterial Proteins/*chemistry/genetics/*metabolism; Protein Biosynthesis; Protein Stability
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:%G eng
Identifiers:ISSN:1365-2958 (Electronic) 0950-382X (Linking) %R MMI7... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/20374493 [ID No:2]