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717325.0 [No comment] 06.04.2016 13:46 Released

ID: 717325.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
The endothelial transcription factor ERG promotes vascular stability and growth through Wnt/beta-catenin signaling
Authors:Birdsey, G. M.; Shah, A. V.; Dufton, N.; Reynolds, L. E.; Osuna Almagro, L.; Yang, Y.; Aspalter, I. M.; Khan, S. T.; Mason, J. C.; Dejana, E.; Gottgens, B.; Hodivala-Dilke, K.; Gerhardt, H.; Adams, R. H.; Randi, A. M.
Date of Publication (YYYY-MM-DD):2015-01-12
Title of Journal:Dev Cell
Issue / Number:1
Start Page:82
End Page:96
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Blood vessel stability is essential for embryonic development; in the adult, many diseases are associated with loss of vascular integrity. The ETS transcription factor ERG drives expression of VE-cadherin and controls junctional integrity. We show that constitutive endothelial deletion of ERG (Erg(cEC-KO)) in mice causes embryonic lethality with vascular defects. Inducible endothelial deletion of ERG (Erg(iEC-KO)) results in defective physiological and pathological angiogenesis in the postnatal retina and tumors, with decreased vascular stability. ERG controls the Wnt/beta-catenin pathway by promoting beta-catenin stability, through signals mediated by VE-cadherin and the Wnt receptor Frizzled-4. Wnt signaling is decreased in ERG-deficient endothelial cells; activation of Wnt signaling with lithium chloride, which stabilizes beta-catenin levels, corrects vascular defects in Erg(cEC-KO) embryos. Finally, overexpression of ERG in vivo reduces permeability and increases stability of VEGF-induced blood vessels. These data demonstrate that ERG is an essential regulator of angiogenesis and vascular stability through Wnt signaling.
Free Keywords:Animals; Antigens, CD/genetics/metabolism; Blotting, Western; Cadherins/genetics/metabolism; Cells, Cultured; Chromatin Immunoprecipitation; Endothelium, Vascular/*cytology/*metabolism; Female; Frizzled Receptors/metabolism; Human Umbilical Vein Endothelial Cells/cytology/metabolism; Humans; Integrases/metabolism; Lung/cytology/metabolism; Mice; Mice, Transgenic; *Neovascularization, Physiologic; Oncogene Proteins/*physiology; Pregnancy; Real-Time Polymerase Chain Reaction; Signal Transduction; Transcription Factors/*physiology; Vascular Endothelial Growth Factor A/genetics/metabolism; Wnt Proteins/genetics/*metabolism; beta Catenin/genetics/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Centre for Tumour Biology, Barts Cancer Institute - a CR-UK Centre of Excellence, John Vane Science Centre, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. Vascular Biology Laboratory, London Research Institute, Cancer Research UK, London WC2A 3PX, UK. FIRC Institute of Molecular Oncology Foundation, IFOM, 20139 Milan, Italy. Department of Haematology, Wellcome Trust and MRC Cambridge Stem Cell Institute and Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB2 0XY, UK. Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine and Faculty of Medicine, University of Munster, D-48149 Munster, Germany. National Heart and Lung Institute (NHLI) Vascular Sciences, Hammersmith Hospital, Imperial College London, London W12 0NN, UK. Electronic address: a.randi@imperial.ac.uk.
Identifiers:ISSN:1878-1551 (Electronic) 1534-5807 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/25584796 [ID No:2]