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Document Version Version Comment Date Status
732957.0 Automatic journal name synchronization 13.05.2017 20:15 Released

ID: 732957.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Origin of a folded repeat protein from an intrinsically disordered ancestor
Authors:Zhu, H.; Sepulveda, E.; Hartmann, M. D.; Kogenaru, M.; Ursinus, A.; Sulz, E.; Albrecht, R.; Coles, M.; Martin, J.; Lupas, A. N.
Date of Publication (YYYY-MM-DD):2016-09-13
Title of Journal:eLife
Sequence Number of Article:e16761
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Repetitive proteins are thought to have arisen through the amplification of subdomain-sized peptides. Many of these originated in a non-repetitive context as cofactors of RNA-based replication and catalysis, and required the RNA to assume their active conformation. In search of the origins of one of the most widespread repeat protein families, the tetratricopeptide repeat (TPR), we identified several potential homologs of its repeated helical hairpin in non-repetitive proteins, including the putatively ancient ribosomal protein S20 (RPS20), which only becomes structured in the context of the ribosome. We evaluated the ability of the RPS20 hairpin to form a TPR fold by amplification and obtained structures identical to natural TPRs for variants with 2-5 point mutations per repeat. The mutations were neutral in the parent organism, suggesting that they could have been sampled in the course of evolution. TPRs could thus have plausibly arisen by amplification from an ancestral helical hairpin.
Free Keywords:Tpr; amplification; ancient peptides; computational biology; protein evolution; protein folding; ribosomal protein; systems biology
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
Identifiers:ISSN:2050-084X (Electronic) 2050-084X (Linking) %R 10.7... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27623012 [ID No:2]